Kedde reagent

Note In the second spray potassium hydroxide solution can be replaced by sodium hydroxide solution or by a solution of 17 g benzyltrimethylammonium hydroxide in 100 ml 33 percent methanol [4]. The Kedde reagent [13] can also be applied very sue-... 

Kedde reagent Solution I 2% of 3,5-dinitrobenzoic acid in MeOH solution II 5.7% KOH in water. Add one drop of each solution to 0.2-0.4 mL of the sample solution, and a bluish to purple color will appear within 5 min. The solution should not contain acetone, which gives a deep bluish color. 

S S-Dinitrobenzoie acid (Kedde reagent) for cardiac glycosides. 

Kaempferol glucoside la 323 Kedde s reagent lb 236,237 1 -Kestose lb 423,424 6-Kestose lb 423 Ketazon lb 280 Keto acids la 262 a-Keto acids la 249,262,372 -, quinoxalone derivatives lb 343... 

Acetogenin derivatives Annona cherimola SiOj CHCI3 - MeOH Kedde s reagent Identification 62 0 CfQ... 

Fig. 5A,B Nerii (oleandri) folium (1). The cardenolides reveal in the R, range 0.1-0.9 a minimum of 13 Kedde po.silive red-violet zones (vis.) or up to 16 blue fluorescent zones after CTA reagent in UV-365 nm, with oleandrin (Tl/R, — 0.85) and adynerin (T3/R — 0.7.5) a.s major cardenolides in the upper R, range. Tlie cardenolide zones in the R, range 0,1-0.8 are due to glycosides of oleandrigcnine, digoxigenine and oleagenine, a.s shown in the following table ...

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