Kallikrein-trypsin inhibitor - Aprotinin

Therapeutic Function Proteinase inhibitor Chemical Name Trypsin inhibitor, pancreatic basic Common Name Aprotinin Frey inhibitor Kallikrein-trypsin inhibitor Chemical Abstracts Registry No. 9087-70-1... 

Aprotinin is a polypeptide consisting of 58 amino acid residues derived from bovine lung tissues and shows inhibitory activity toward various proteolytic enzymes including chymo-trypsin, kallikrein, plasmin, and trypsin. It was also one of the first enzyme inhibitors used as an auxiliary agent for oral (poly)peptide administration. The co-administration of aprotinin led to an increased bioavailability of peptide and protein drugs [5,44,45], The Bowman-Birk inhibitor (71 amino acids, 8 kDa) and the Kunitz trypsin inhibitor (184 amino acids, 21 kDa) belong to the soybean trypsin inhibitors. Both are known to inhibit trypsin, chymotrypsin, and elastase, whereas carboxypeptidase A and B cannot be inhibited [7,46],... 

Studies by many workers have revealed two peptide sequences which are very selective for thrombin -Phe-Val-Arg- and -X-Pro-Arg- (C5, S24, S26). As can be seen in Table 3, these two sequences have been widely used to design the various synthetic substrates to detect thrombin. This measurement can be done directly by incubating this enzyme with the appropriate substrate. Latallo has recommended adding an agent like aprotinin or soybean trypsin inhibitor to prevent attack by kallikrein, Factor Xa, or plasmin on these thrombin substrates (L5). The use of the original substrate, S-2160, has sharply declined due to its lower sensitivity, specificity, and solubility when compared to substrates like S-2238 and Chromozyn TH. Today,... 

Serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein, and plasmin Binding is reversible, with most aprotinin-protease complexes dissociating at pH>10or <3 Peptidase inhibitor... 

Tobacco-produced recombinant research-grade bovine aprotinin (Apronexin NP) [238] is also available from Sigma Aldrich. Aprotinin is a protease inhibitor which is used as a research reagent in biomanufacturing for several therapeutic applications. It has been traditionally extracted from bovine lung tissue. Aprotinin is a single, 58 amino-acid polypeptide with three disulfide bonds, and inhibits several serine proteases such as trypsin, chymotrypsin, plasmin, and kallikrein. 

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