Jumping-PCR

Giy4-Ser)3 linker Forward for jumping-PCR-assembiy LinkerFor 5 AGA TCC GCC GCC ACC CGA CCC 3 ... 

Set up two sets of jumping PCR reactions, the first for the VH products involving nine reactions (one for each trf the nine primary PCR reactions from Protocd 5) and the second for the VK products involving eight reactions (one for each of the ei t primaiy PCR reactions from Protocol 5). For each of the 17 jumping PCR reactions, set up the following 100 pi mixture ... 

For jumping PCR, we recommend using AmpliTaq DNA polymerase which in our hands works better than Deep VentR DNA pol5nnerase. 

Estimate the quantities of VH-linker and linker-VL DNA prepared by the jumping PCR reactions (Protocol 7) using a dot spot technique." Adjust the concentrations of VH-linker and linker-VL PCR products to 5 ng/jcl. 

Let us first consider a network immersed in a melt of polymer chains with degree of polymerization p. In the athermal case, the network should be swollen. As polymer-network interaction parameter Xnp increases, the volume of the network decreases until a practically complete segregation of the gel from polymer melt occurs. It has been found [34, 35] that two qualitatively different regimes can be realized either a smooth contraction of the network (Fig. 8, curve 1) or a jumpwise transition (Fig. 8, curve 2). The discrete first order phase transition takes place only for the networks prepared in the presence of some diluent and when p is larger than a critical value pcr m1/2. The jump of the... 

If network chains carry charged units, the coil-globule transition becomes sharper the amplitude of the jump increases, the critical value of pcr decreases down to unity pcr 1 (see Fig. 10). 

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