Jones, Mary Ellen

Volume LI. Purine and Pyrimidine Nucleotide Metabolism Edited by Patricia A. Hoffee and Mary Ellen Jones... 

Departments of Ophthalmology and Visual Sciences, Molecular Biology and Pharmacology, Washington University Medical School, CB 8096, 660 S. Euclid Avenue, St Louis, MO 63110, and %Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, 405 Mary Ellen Jones, CB 7260, Chafe I Hill, NC, 27599, USA... 

Mary Ellen Jones and her colleagues set out to purify the enzyme or enzymes involved in these two reactions. Their main goal was to determine whether the two reactions are carried out by one protein or more than one. Their findings indicated that the two reactions were both catalyzed by the same enzyme, consisting of a single polypeptide chain. To demonstrate this fact, it was necessary to monitor both enzyme activities at each step in the purification and show that both activities copurified. For this purpose, Jones used specific enzyme assays for both enzyme activities. All fractions were assayed for both enzymatic activities at each stage of the purification. 

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