Isotopics derivatives, spectroscopy synthesis

Probably, one of the most valuable advances in this field has dealt with the first chemoenzymatic synthesis of the stable isotope-enriched heparin from a uniformly double labelled 13C, 15N /V-acetylheparosan from E. coli K5. Heteronuclear, multidimensional nuclear magnetic resonance spectroscopy was employed to analyze the chemical composition and solution conformation of N-acety 1 hcparosan, the precursors, and heparin. Isotopic enrichment was found to provide well-resolved 13C spectra with the high sensitivity required for conformational studies of these biomolecules. Stable isotope-labelled heparin was indistinguishable from heparin derived from animal tissues and might be employed as a novel tool for studying the interaction of heparin with different receptors.30... 

Through-bond and through-space solid-state NMR correlation experiments including CP-INADEQUATE at natural isotope abundance have been applied by Brus and Jegorov to establish the structure and dynamic behaviour of simvastatin (C25H38O5), an active metabolite which inhibits 3-hydroxy-3-methyl-glutaryl coenzyme A, (HMG-CoA) reductase, the enzyme that is necessary in an early step of the cholesterol synthesis. 2D INADEQUATE spectroscopy has been applied by Meier et to establish the structures of a series of fullerene derivatives. 

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