Isotopes sugars labeled with

The employment of NMR-active isotopes permits to access experimental parameters which are intrinsically difficult to measure, unless a significant concentration of the sugar is present in the NMR tube. For instance, aqueous solutions of N-acetyIncuraminic acid, labeled with 13C at Cl, C2, and/or C3, were analyzed to detect and quantify the various chemical species present in equilibrium at different pHs. In fact, in addition to the expected a and (3 pyranose forms, acyclic keto, keto hydrate and enol forms were identified on the basis of 13C NMR spectroscopic data. Besides, DFT methods were employed to predict the effect of enol and hydrate structure on the coupling constant values Jc,u and /c c involving C2 and C3, finding that 2/c2,h3 can be safely used to differentiate the cis and tram isomers of the enol forms.9... 

Reduction of aldonolactones and their derivatives with isotopically modified reducing agents leads to sugars labeled at the anomeric center. Glycosides substituted with deuterium or labeled with tritium are widely employed for kinetic isotope-effect measurements, mechanistic studies, isotope-tracing experiments, and so on. 

Hydrogen, isotopes of, sugars specifically labeled with,... 

SUGARS SPECIFICALLY LABELED WITH ISOTOPES OF HYDROGEN ... 

IX. Tables of Known Sugars Specifically Labeled with Isotopes of Hydrogen.. 181... 

Most of the sugars specifically labeled with isotopes of hydrogen that have been synthesized were prepared for use in mechanistic studies of enzymes. 

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