Isomalto-oligosaccharide, production

The final product concentration depends significantly on the ratio of substrate and acceptor. The first acceptor product (panose in the presence of maltose) is favored at high maltose in excess, further tetra- and pentasaccharides are formed in significant amounts.37 Thus isomalto-oligosaccharides (IMOS) can be produced in good yield under appropriate reaction conditions (see Section III). 

The products are those produced by Leuc. mesenteroides B-512F dextransucrase unless otherwise indicated. b The starred products are also acceptors that give a homologous series with a-isomalto-oligosaccharides attached to the acceptor.r Structure not determined.d Two products are formed by Leuc. mesenteroides B-512F dextransucrase. Not an acceptor. Four products are formed by Strep, mutans GTF-I. Product formed by altemansucrase. 

Dextransucrase Leuconostoc mesenteroides Production of isomalto-oligosaccharide beer... 

Both and H n.m.r. measurements on a bacterial dextran and its acetylated derivative and on a synthetic tri-O-benzyldextran have revealed the main features of their linear a-(l - 6)-linked structures. Bacterial dextrans from Streptococcus viridans B-1351, Streptobacterium dextranicum B-1254, and four strains of Leuconostoc mesenteroides have been subjected to methylation analysis.They differed with respect to the ease of methylation and subsequent hydrolysis of the methylated polysaccharides, owing to significant differences in the frequency and type of chain-branching. An enzymic approach has been used to obtain information on the frequency and distribution of chain-branching in dextrans. Thus, B-512 native dextran was treated with an endo-dextranase (from Pseudomonas UQM 733) and the products were examined by quantitative t.l.c. The relative amount of each oligosaccharide (i.e. linear isomalto-oligosaccharides of DP 2-5... 

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