Isoluminol thiocarbamyl derivatives

Obviously, the main purpose for the introduction of CL detection coupled to CE separations is inherent to the development and improvement of sensitive and uncomplicated devices to achieve a decrease of the band broadening caused by turbulence at the column end, together with the attractive separation efficiency of CE setups. With this purpose in mind, Zhao et al. [83] designed a postcolumn reactor for CL detection in the capillary electrophoretic separation of isoluminol thiocarbamyl derivatives of amino acids, because, like other isothiocyanates, isoluminol isothiocyanate has potential applications in the protein-sequencing area. 

The elimination of turbulent mixing in the flow chamber and the short residence time of the reaction mixture in the detection chamber provided a high separation efficiency of 100,000 theoretical plates for labeled amino acids, obtaining good resolution in comparison with previous CL detectors [79, 82], For the isoluminol thiocarbamyl derivative of valine, a detection limit of 500 amol was reported however, it was not possible to separate all 20 isoluminol-deriva-tized amino acids. 

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See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ ]

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