Isoluminol isothiocyanate

ABEI, M(4-ami nobutyl )-Methylisolu mi nol BSA, bovine serum albumin CL, chemiluminescence DNPO, tas-(2,4-dinitrophenyl)oxalate ECL, electrogenerated chemiluminescence EMMA, electrophoretically mediated microanalysis EY, eosine Y FR, lluorescamine HRP, horseradish peroxidase ILITC, isoluminol isothiocyanate LOD, limit of detection RITC, rhodamine B isothiocyanate TCPO, Mv-(2,4,6-trichlorophenyl)oxalate TEA, triethylamine TRITC, tetramethylrhodamine isothiocyanate. 

Obviously, the main purpose for the introduction of CL detection coupled to CE separations is inherent to the development and improvement of sensitive and uncomplicated devices to achieve a decrease of the band broadening caused by turbulence at the column end, together with the attractive separation efficiency of CE setups. With this purpose in mind, Zhao et al. [83] designed a postcolumn reactor for CL detection in the capillary electrophoretic separation of isoluminol thiocarbamyl derivatives of amino acids, because, like other isothiocyanates, isoluminol isothiocyanate has potential applications in the protein-sequencing area. 

In the first reported example of chemiluminescence energy transfer, the iso-luminol derivative ABEI (Fig. 14) was covalently attached via its isothiocyanate to either rabbit immunoglobulin or cyclic AMP. Antibodies to these molecules were labeled with fluorescein. Homogeneous competitive immunoassays were conducted for both analytes and, in order to maximize the combined effects of fluorescein emission and isoluminol quenching, the parameter measured, as a function of analyte concentration, was the intensity ratio at the wave-... 

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