Isolation of active compounds

The use of natural products as medicine has invoked the isolation of active compounds the first commercial pure natural product introduced for therapeutic use is generally considered to be the narcotic morphine (1), marketed by Merck in 1826, and the first semi-synthetic pure dmg aspirin (2), based on a natural product salicin (3) isolated from Salix alba, was introduced by Bayer in 1899. This success subsequently led to the isolation of early dmgs such as cocaine, codeine, digitoxin (4), quinine (5), and pilocarpine (6), of which some are still in use. ... 

CareU T, Wintner EA, Rebek J Jr. A solution-phase screening procedure for the isolation of active compounds from a library of 43. molecules. Angew. Chem. Int. Ed. 1994 33 2061-2064. 

Rebek and coworkers have also recently published a procedure for the synthesis of libraries of small organic molecules, and have designed a screening method for the isolation of active compounds from such libraries. A trypsin inhibitor with a X of 9 pM was found from the screening of a library [97]... 

NATURAL PRODUCTS IN E. Isolation of active compounds H. Antimalarial agents... 

It can be concluded from this review that a whole range of low molecular weight compounds exhibits complement-modulating properties. There is little doubt that the use of complement-based assays to guide the isolation of active compounds from plants and plant extracts will add more natural products with a variety of structures to this list. 

Bioassay-guided fractionation led to the isolation of active compounds. 

Extraction of crude extracts is the first and the most crucial step in the procedure for the isolation of active compounds from TCMs. A pipeline for the extraction process is composed of the following steps ... 

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