Isoenzymes ribonuclease

Many of the nucleic acid hydrolases occur in isoenzymic forms in mammalian tissues. Some of these isoenzymes are glycoenzymes, and information on these is contained in Table I. Ribonuclease B has been studied in the greatest detail. Following the initial observation that the ribonuclease from bovine pancreas occurs in isoenzymic forms,45 it was found that one form (ribonuclease B) contains an appreciable... 

Ribonuclease from other mammalian sources may have several carbohydrate fragments in their structures. Thus, ribonuclease B from porcine pancreas contains at least three carbohydrate moieties, and these are attached9 at asparagine residues 21, 34, and 76. The carbohydrate moieties present at residues 21 and 76 are considerably more complex than that at residue 34, and their structures are under investigation in several laboratories. The carbohydrate portions of the isoenzymes of deoxyribonuclease are similar in structure to those from the ribonucleases.11 As ribonuclease and deoxyribonuclease originate in the same organ, it is possible that the same pathways and enzymes are utilized for the biosynthesis of the carbohydrate moieties of both enzymes. 

Purification of bull seminal plasma hyaluronidase Purification of ribonuclease B Purification of biliary glycoprotein I Separation of human amylase isoenzymes Purification of cathepsin D from rat spleen Purification of a cathepsin E-like acid proteinase from rat spleen... 

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