Isoelectric integral

Chen, J., Lee, C.S., Shen, Y., Smith, R.D., Baehrecke, E.H. (2002). Integration of capillary isoelectric focusing with capillary reversed-phase liquid chromatography for two-dimensional proteomics separation. Electrophoresis 23, 3143-3148. 

The self-assembly of caseins may be readily manipulated by processing methods that affect the integrity of native casein micelles and the character of the casein interactions in aqueous media. Examples of such procedures are (Dickinson, 2006) (i) acidification toward the isoelectric point (p/) (pH 4.6-4.8), leading to a neutralization of the net protein charge (ii) enzyme action, as exploited in the production of cheeses and fermented milks (iii) addition of divalent ions, especially, Ca2+ ions (iv) addition of sucrose or ethanol (v) temperature treatment and (vi) high-pressure treatment. 

Li, Y., Buch, J.S., Rosenberger, F., DeVoe, D.L., Lee, C.S., Integration of isoelectric focusing with parallel sodium dodecyl sulfate gel electrophoresis for multidimensional protein separations in a plastic microfluidic network. Anal. Chem. 2004, 76, 742-748. 

An integrated treatment of isoelectric focusing and isopycnic sedimentation was presented in 1971 [50] the following is based on that treatment. 

Focusing Isoelectric focusing is an integral part of the two-dimensional analysis of... 

Y.J. Wang, B.M. Balgley, P.A. Rudnick, E.L. Evans, D.L. DeVoe and C.S. Lee, Integrated capillary isoelectric focusing/nano-reversed phase hquid chromatography coupled with ESI-MS for characterization of intact yeast proteins. Journal of Proteome Research, 4, 36 2 (2005). 

---