IR spectroscopic imaging

Max Diem, Melissa J. Romeo, Susie Boydston-White 

For the analysis of exfoliated cells (such as an automated Pap test for cervical cancer screening), the ability to collect the spectrum of each individual cell separately by mapping methods is even more important, since statistical analysis 

In other sections of this volume,6 detailed discussions on the principles of data acquisition for IR spectral imaging are presented (Chapter 1, this volume). Here, we shall restrict ourselves to a description of the instrumentation used for the research described in the following review. 

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It is clear that the introduction of the IR FPA detector has brought Fourier transform infrared (FTIR) microscopy with a thermal source to a new and exciting stage of development. This is illustrated in the other chapters of this volume. Our purpose in this chapter is to address how IR FPA technology could be combined with the synchrotron source to advance IR spectroscopic imaging in ways that would prove quite difficult with a conventional thermal source. To address this question, we will need to understand the detailed nature of the synchrotron IR source, the optical... 

Figure 5.9 IR spectroscopic images of phase separation of PBD—DAP blends (50 wt.% DAP) taken immediately after sample preparation. Reproduced from figure 2 of Ref. 27, with permission.

Figure 5.10 IR spectroscopic images of phase separation of PBD-DAP blends (50 wt.% DAP) taken 24 h after sample preparation. The images are reported (a) at 2950, (b) 1730 and (c) 1280 cm-1. DAP domains in the PED matrix and interfacial regions are observed. Reproduced from figure 2 of Ref. 27, with permission.

Materials analysis systems based on real-time near-IR spectroscopic imaging... 

IR spectroscopic imaging offers the advantage of fast measurements with lateral resolution of a few micrometers. Due to the high acquisition rate of spectral data even dynamic systems can be studied at a time resolution of seconds. This report will demonstrate the unique opportunities by FTIR spectroscopic imaging to investigate material characterized by small thickness of the sample layer and non-crystalline state. SPR imaging is a new technique based on the classical SPR that combines high sensitivity with excellent lateral reso-... 

R. (2006) Identification of primary tumors of brain metastases by SIMCA classification of IR spectroscopic images. Eiochim. Eiopkys. Acta, 1758, 883-91. 

Kazarian, S.G. and Chan, K.LA. (2006) Application of ATR-FT-IR spectroscopic imaging to biomedical samples. Biochim. Biophys. Acta, 1758, 858-67. 

A three-dimensional multivariate image processing technique for the analysis of FT-IR spectroscopic images of multiple tissue section. BMC Med. Imaging, 6, 12. 

Lasch, P. (2005) CytoSpec 1.2-an Application for FT-IR Spectroscopic imaging see http //www.cytospec.com (accessed 27 November 2008). 

Observation of a Penetration Depth Gradient in ATR FT-IR Spectroscopic Imaging Applications... 

The above-discussed examples demonstrate that FT-IR spectroscopic imaging can be applied in a multiplicity of applications for the characterization of polymers. 

Fig. 24 IR spectra of poly(vinyl pyrrolidone) and poly(vinyl methyl ether). The gray areas indicate the spectral ranges which were used to calculate the contrast of the FT-IR spectroscopic images in Fig. 23...

C. Marcott, R. C. Reeder, J. A. Sweat, D. D. Panzer, D. L. Wetzel. FT-IR spectroscopic imaging microsocopy of wheat kernels using a mercury-cadmium-telluride focal-plane array detector. Vibrat Spectrosc 19 123-129, 1999. 

FT-IR spectroscopic imaging microscopy of wheat kernels using a Mercury-Cadmium-Telluride focal-plane array detector. Vib. Spectrosc., 19 (1),... 

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