IR Spectromicroscopy

It is also worth noting that there are efforts underway to develop near-field IR spectromicroscopy to further improve the spatial resolution perhaps to a fraction of the diffraction limit (Keilmann 1999 Talley 2000). 

Figure 4. Optical image and two infrared images of a dividing cell (35 pm x 20 pm) obtained from IR spectromicroscopy. These chemical maps are derived from the strength of absorption bands from approximately 100 infrared transmittance spectra collected over the area of the dividing cell. The maximum in the color scale (yellow) represents the position of the maximum absorption by the cell, while the minimum (blue) represents no absorption by the cell. Strikingly, the intensity map of the amide II absorption band shows two peaks in the center of the two halves of the cell, representing the position of two separate nuclei before the cell division is complete. The intensity map of the C-H stretch absorption bands shows that lipids are concentrated at the contractil ring, where the cleavage furrow is located. [Used by permission of the National Academy of Sciences, U.S.A., from Jamin et al. (1998), Proc Natl Acad Sci, Vol. 95, Fig. 3, p. 4839.]...

In addition to IR spectromicroscopy it is possible to use IRSR to probe molecular interactions at single crystal surfaces. The chemical and physical properties of the surfaces of solid materials and the interfaces between solids and fluids play an... 

Infrared Synchrotron Radiation Beamlines High Brilliance Tools for IR Spectromicroscopy... 

The biaxial orientation in photoaddressable azobenzene films has been observed recently by polarized Raman spectromicroscopy [64]. Here, IR spectroscopy has been advantageously used as a complementary technique to measure the order... 

The analysis of MDA-MB-231 cells incubated with 53 was also carried out on a synchrotron-based multiple beam FT-IR imaging (IRENI) set up at the Synchrotron Radiation Center, Stroughton, Wisconsin, equipped with a FPA detector of 96 X 96 pixels, where each pixel corresponds to a 0.54 x 0.54 pm area of the sample. With the better resolution afforded by this technique, the complex appears heterogeneously distributed in the cell, but with a higher perinuclear concentration [79]. In addition, 53 was mapped in cell by SR-UV-SM (synchrotron radiation UV spectromicroscopy), confocal Raman microscopy, and AFM-IR (see Sections 11.3.3 and 11.3.4). Thus, this innovative family of complexes appeared to be a valuable multimodal (and not only bimodal) tool for cell imaging. 

Synchrotron-Based Infrared Raster-Scanned (IR SR) Spectromicroscopy... 

Figure 15.7 Comparison of RS and WF spectromicroscopy for Cicada Wing. Chemical intensity maps of amide II and CHj absorbance bands obtained by integration of the respective bands (amide II 1588-1483 cm-, v,5 CHj 2931 2913 cm- ), (a), (b), and (c) represent the images obtained from the IR scans performed at the RS spectromicroscopy beamline at the Australian Synchrotron while (d) to (i) are the respective images obtained from the WF spectromi-crscopy IRENI beamline. The intensities of the integrated area of the amide II band (a, d, and g) and CHj antisymmetric stretching (b, e, and h) are shown in consistent color...

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