Ionic strength, micellar electrokinetic

Another format of chromatography which has been performed on electrophoresis chips is micellar electrokinetic capillary chromatography (MECC). This technique represents a very powerful extension of CE for the separation of both neutral species and ionic compounds, and has been originally developed by Terabe et al. [76]. Transfer of MECC to CE microchips has been first demonstrated by Moore et al. [77]. The experimental procedure used is identical to high speed CE, apart from the fact that a MECC buffer with 50 mM SDS and 10% methanol was used. Again three coumarin dyes were used as a model sample. Separation was achieved within a few minutes. At low applied field strengths (< 400 V/cm), the reproducibility was found to be excellent (below 1 %). 

FAS can be implemented on microchips in a very similar manner as capillary electrophoresis. However, the requirement of low ionic strength sample buffer for FAS puts limitation on its use as general preconcentration technique. Variations of the technique, such as transient isotacho-phoresis and micellar electrokinetic sweeping, have been more successfully used. Jung et al. reported (Mi-chip transient isotachophoresis... 

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