Ion-exchange chromatography principles

Rossomando EF (1990) In Deutscher M P (ed.) Guide to protein purification (Meth Enzymol Vol. 182). Academic Press, San Diego, p 309 Amersham Biosciences (2004) Ion exchange chromatography Principles and methods. ISBN 91 970490-3-4. www.chromatography.amershambiosciences.com... 

Ion exchange chromatography Principles and Methods, Amersham Biosdence. 

Ion-Exchange Chromatography, Principles and Methods, Pharmacia Biotech, Sweden. 

Ion-exchange chromatography principles and methods. GE Health Care, http //www6. gelifesciences.com/APTRIX/upp00919.nsf/ content /handb o oks. 

Amersham Pharmacia Biotech (ed), Ion Exchange Chromatography Principles and Methods, APB, Uppsala, 1999. 

Ion Exchange Chromatography - Basic principles of ion exchange chromatography and studies conducted from Texas A M University. http //ntri.tamuk.edu/fplc/ion.html. 

Figure 6.11 Principle of ion-exchange chromatography, in this case anion exchange chromatography. The chromatographic beads exhibit an overall positive charge. Proteins displaying a nett negative charge at the pH selected for the chromatography will bind to the beads due to electrostatic interactions...

FIGURE 6.4 Schematic illustration of the principle of ion exchange chromatography. 

In principle, an extraction technique involving pH adjustment of the aqueous phase can offer purification similar to ion exchange chromatography. Although the method uses a smaller volume of solvent, it has limited ability to remove low-level impurities. Therefore, the replacement of ion exchange chromatography with extraction requires some ingenuity. 

Illustration of the principles of ion-exchange chromatography. See text for explanation. 

A rigorous mathematical treatment of ion-exchange chromatography would be rather complex and well beyond the scope of this text. A brief description of the principles of ion-exchange chromatography is given below. 

Karlsson E, Ryden L, Brewer J (1998), Ion exchange chromatography, In Janson JC, Ryden L (Eds), Protein Purification Principles, High-Resolution Methods, and Applications, 2nd Ed., Wiley-VCH, Weinheim, pp. 145-206. 

This simple exercise demonstrates the chromatographic separation of the components of a mixture by a procedure that relies on two of the principles discussed above, namely ion-exchange chromatography and size-exclusion chromatography. The components to be separated (see Table 2-3) differ greatly from one another both in their molecular... 

Background and principles Ion-exchange chromatography is a form of adsorption chromatography lhal relies on Ihe ionic and eleclroslalic properties of charged... 

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