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Ion-Exchange Chromatography IEC

In ion-exchange chromatography (IEC), the mobile phase modulator is typically a salt in aqueous solution, and the stationary phase is an ion-exchanger. For dilute conditions, the solute retention factor is commonly found to be a power-law function of the salt normality [cf. Eq. (16-27) for ion-exchange equilibrium]. [Pg.45]

Hydrophobic interaction chromatography (HIC) occupies a unique niche in the field of analytical chromatography. A particular advantage of HIC is its unique selectivity. Whereas ion-exchange chromatography (IEC) principally reveals differences based on the surface charge of native proteins, HIC reveals differences based principally on their surface hydrophobicity. HIC is complementary to reversed-phase chromatography (RPC) in a different sense. Whereas HIC discriminates primarily on the basis of surface hydrophobicity, RPC principally reveals differences based on total hydrophobicity of all the hydrophobic residues of denatured proteins. [Pg.81]

Ion-exchange chromatography (IEC), on the basis of differences in the pKa values of the functional groups of the side chains resulting in changes in the ionization function of pH... [Pg.133]

In ion-exchange chromatography (IEC), species are separated on the basis of differences in electric charge. The primary mechanism of retention is the electrostatic attraction of ionic solutes in solution to fixed ions of... [Pg.38]

Ion Exchange Chromatography. In ion exchange chromatography (IEC), the solid stationary phase is comprised of ionic material. This serves as an ion exchanger and separation is achieved primarily on the basis of the relative affinities of... [Pg.85]

The molecular characteristics exploited for the separation of proteins and peptides include molecular size (size exclusion chromatography, SEC), net charge (ion-exchange chromatography, IEC), polarity (reverse-phase chromatography, RPC), hydrophobicity (hydrophobic interaction... [Pg.267]

One of the most popular LC techniques used in grape and wine protein analysis is Ion Exchange Chromatography (IEC), which is based... [Pg.268]

See other pages where Ion-Exchange Chromatography IEC is mentioned: [Pg.109]    [Pg.11]    [Pg.214]    [Pg.287]    [Pg.173]    [Pg.705]    [Pg.428]    [Pg.108]    [Pg.313]    [Pg.367]    [Pg.53]    [Pg.1046]    [Pg.1086]    [Pg.59]    [Pg.67]    [Pg.181]    [Pg.73]    [Pg.107]    [Pg.136]    [Pg.190]    [Pg.376]    [Pg.402]    [Pg.3]    [Pg.215]    [Pg.388]    [Pg.82]    [Pg.82]    [Pg.110]    [Pg.241]    [Pg.84]    [Pg.255]    [Pg.515]    [Pg.546]    [Pg.16]    [Pg.68]    [Pg.29]   
See also in sourсe #XX -- [ Pg.82 , Pg.83 , Pg.84 , Pg.85 , Pg.86 , Pg.87 , Pg.88 , Pg.89 , Pg.90 , Pg.91 , Pg.92 ]



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