Iodogen, labelling with

Antimyosin Fab was labeled with 125I and 123I by the Iodogen iodination method (61) because of its gentler nature of compared to chloramine T. Free and AM-bound radioactivity were separated as described above. 

Peroxidases Enzymatic labeling is a milder alternative to chemical oxidants. It can be accomplished without deactivation or degradation. It is thus suitable to label very sensitive molecules, such as biomolecules. The labeling yields are close to those obtained with chloramine-T and iodogen, and no carrier-added radioiodination can be obtained (Davidson, 1987). 

Pierce iodination-labeling tubes (Thermo Fisher Scientific), glass tubes coated with iodogen reagent, the labefing reaction takes place at the surface of the water-insoluble oxidant. 

The chemistry of iodine radionuclides used in nuclear medicine has been extensively explored. Isotopic exchange procedures, direct iodination approaches (those using chloramine-T and iodogen), and indirect approaches (the Bolton-Hunter method) are but a few of the well-known synthetic strategies to label biomolecules with iodine (24). Some of the most important 1-labeled imaging tracers [1-labeled iodoazomycin arabinoside ( lAZA), 1-labeled vasoactive intestinal peptide ( 1-VlP), etc.] will be discussed in the following sections. 

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