Interactions with lentil lectins

Hemagglutinating activity in the common lentil (Lens culinaris or Lens esculenta) was first reported by Landsteiner and Raubitschek.439 Several laboratories have since isolated the lentil lectin in pure form, and studied its physical-chemical properties and interaction with carbohydrates.138,142,143,440 442... 

Like the jack-bean and pea lectins, the lentil agglutinin interacted with 2-0-(2-acetamido-2-deoxy-j8-D-glucopyranosyI)-D-mannose,210 providing evidence that the lentil lectin binds to internal 2-0-substituted D-mannopyranosyl residues that occur in animal glycoproteins. 

Inasmuch as extensive maleylation of free amino groups did not abolish the hemagglutinating and polysaccharide-precipitating activity of the lentil lectin, it is probable that amino groups are not involved in the carbohydrate-binding site of the lectin.4473 Treatment of the lentil lectin with 1-acetylimidazole resulted in the modification of 3-5 tyrosyl and 7-17 amino groups.4473 The modified proteins had lost their hemagglutinating activity, but retained their capacity to interact with polysaccharides and to bind to erythrocytes.4473... 

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