Interactions of Proteins with Oxides

Nuclear Magnetic Resonance Studies of Interfacial Phenomena 

The protein backbone is situated at the periphery of the clay mineral particles while the side chains enter in the layers. Surface area and pore volume decreased. The morphology of the particles was changed. Al NMR analysis showed that the tetrahedral A1 species are involved in the adsorption process whereas the octahedral sites are influenced by grafting. 

Betega de Paiva et al. (2008) analyzed properties, synthesis, and applications of organoclays (especially bentonite and polymer nanocomposites), modifications with several chemical compounds as quaternary alkylammonium salts and also biomolecules as enzymes that provide applicability in a variety of fields. 

This could affect the structure of the adsorption complexes of HPF on both hydrophilic and hydro-phobic surfaces (Tunc et al. 2005). 

Interaction of HPF with nanosilica is of special interest because of the medical applications of this material, especially as a component of tourniquet preparations. Therefore, here we will analyze adsorption interactions of fibrinogen with nanosilica depending on concentration of components, salinity, and pH. Application of several methods such as (i) adsorption of HPF from the aqueous solutions (ii) UV and FTIR spectroscopy of adsorbed layer with HPF and (iii) TSDC and H NMR spectroscopy with layer-by-layer freezing-out of bulk and bound water interacting with HPF and HPF/nanosilica allows us to deeply understand features of interaction of HPF with silica NP that is of importance for elucidation of the mechanism of interaction of nanosilica preparations with blood. 

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