Interaction of Radiation with the Flowing Sample

Images of broken light which dance before me like a million eyes 

Flow Analysis with Spectrophotometric and Luminometric Detection 

The different interactions of radiation with the flowing sample in relation to the different spectrophotometric techniques are presented below. Basic aspects are dealt with in relation to a static cuvette, whereas those aspects more related to flow analysis are discussed in relation to a flowthrough cuvette. 

UV—Visible spectrophotometry relies on the wavelength dependent absorption of electromagnetic radiation by the processed sample. The 

When a monochromatic beam of radiation traverses a cuvette containing the processed sample (Fig. 4.1) and some of the radiation is absorbed (ideally, this is the only process occurring), the power of the incident light beam (Pi) is reduced to Pe, the power of the emergent beam the ratio Pe/Pi is known as the transmittance (T). The absorbance (A) is defined as 

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