Inter- and Intrastrand DNA Cross-Links

Fusion of the purine/pyrimidine rings either as intra- or interstrand cross-links can occur upon exposure to ionizing radiation, chemotherapeutic agents (e.g., cisplatin), or photosensitization reactions (psoralens), or from endogenous generation of bisfunctional enals mentioned earlier (e.g., acrolein, crotonaldehyde, and 

Analysis of the Pol T apo and ternary structures reveals some important features of Y-family catalysis. Binding of DNA and the incoming dNTP results in the thumb 

Much more information is available from kinetic analyses, particularly since it has become possible to begin relating this information to structure. Earlier con- 

Compilation and alignment of DNA polymerase sequences. Nucleic Acids Res., 19, 4045 1057. 

and Kornberg, A. (1958) Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction./. Biol. Chem., 233,171-177. 

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Platinum agent forms inter-and intrastrand DNA cross-links inhibition of DNA synthesis aquated species is cytotoxic... 

The polynuclear platinum compounds stand in vivid contrast to mononuclear platinum complexes because the predominant DNA lesions are long-range inter- and intrastrand cross-links where the sites of platination may be separated by up to four base pairs. The consequent structural and conformational changes in DNA are also distinct. 

Sanchez, A.M., Kozekov, I.D., Harris, T.M., and Uoyd, R.S. (2005) Formation of inter- and intrastrand imine type DNA-DNA cross-links through secondary reactions of aldehydic adducts. Chem. Res. Toxicol, 18, 1683-1690. 

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