Intensity diagrams for mechanistic information

During the progress of the reaction fluorescence reaction spectra are taken and evaluated in the same way as the absorption spectra. First, fluorescence spectra are recorded at the chosen reaction times, and the intensities at wavelengths with significant changes are plotted versus the reaction time yielding intensity-time diagrams. 

These diagrams allow the determination of the number and the appearance of fluorescent compounds. The variety of the forms of these diagrams is manifold and more characteristic than in absorbance diagrams. As can be seen in Fig. 5.38, even for the same mechanism the fluorescence diagrams are extremely dependent on the wavelength of observations. 

Different types of diagrams influenced by differences in fluorophore properties as well as diagrams of high order in comparison to absorbance quotient diagrams are discussed in detail elsewhere [92]. 

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