Intact secretory cells

LASER SCANNING CONFOCAL MICROSCOPY OF INTACT SECRETORY CELLS... 

The visible fluorescence of intact living cells is called autofluorescence. If the cells treated with special fluorescent dyes, the cellular common fluorescence also includes the additional light emission. Autofluorescence could be used (i) in express-microanalysis of the accumulation of the secondary metabolites in secretory cells without long biochemical procedures (ii) in diagnostics of cellular damage and (iii) in analysis of cellcell interactions (Roshchina, 2003). During the plant development, secretory... 

Roshchina, V.V., Melnikova, E.V., Mitkovskaya L.I. and Karnaukhov, V.N. (1998). Microspectrofluorimetry for the study of intact plant secretory cells. Journal of General Biology (Russia). 59 531-554. 

Camell, L., and Moore, H.P., 1994, Transport via the regulated secretory pathway in semi-intact PC12 cells role of intra-cistemal calcium and pH in the transport and sorting of secretogranin II. J. Cell Biol. 127, 693-705... 

Mannose, although less effective than glucose, also, stimulates insulin secretion. Fructose, which is not metabolized in the j cell, has little or no effect on insulin secretion. Galactose, xylose, and L-arabinose are ineffective. The glucose effect depends on an intact secretory mechanism and glucose metabolism. 

Pardal R, Ludewig U, Garcia-Hirschfeld J, Lopez-Bameo J. Secretory responses of intact glomus cells in thin slices of rat carotid body to hypoxia and tetraethylammo-nium. Proc Natl Acad Sci USA 2000 97(5) 2361-2366. 

Figure 5 Secretory responses of intact glomus cells exposed to channel blockers, (a) Amperometric recording from a glomus cell illustrating the similar effects elicited by low Poj and the application of 5 mM TEA. (b) Frequency histograms of the quanta charge of events elicited by hypoxia and TEA. Note that the parameters of the distributions are the same in the two experimental conditions, (c) Secretory activity induced in a glomus cell by hypoxia and 200 nM IhTX. (Modified from Ref 17.)...

Omeprazole itself is not the active inhibitor of the H+, K+-ATPase, however. Rather, the transformation of omeprazole in acid is required to inhibit the H+, K+-ATPase in vitro and in vivo, whereas intact omeprazole is devoid of inhibitory action. Isolated H+, K+-ATPase is blocked by omeprazole only after the pretreatment of omeprazole with acid. Conversely, neutralization of the acid-secretory canaliculi of isolated gastric gland and parietal cell preparations by permeable buffers, which blocks the acid-catalyzed transformation of omeprazole, prevents inhibition of the enzyme. Furthermore, in-vivo blockade of acid secretion using an H2-receptor antagonist prior to omeprazole administration decreases the inhibitory potency of omeprazole, whereas the stimulation of acid secretion (e.g., by food intake) increases the potency. 

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