Insoluble acceptor polymers, enzymic

This chapter discusses recent examples of enzymic oligosaccharide synthesis on polymer supports. Examples include cases where water insoluble acceptor-polymers of improved accessibility are used with soluble enzymes, and cases where water-soluble acceptor-polymers are used with either insoluble or soluble enzymes. The methodology is useful for preparative-scale reactions and for the study of enzymic properties. 

Water-soluble Acceptor-Polymers and Insoluble Enzymes... 

Irradiation at >320 nm releases from the polymer, whether insoluble or water-soluble, free oligosaccharides in very high yields. A simple illustration of such a sequence carried out with either insoluble 2-aminoethyl-substituted poly(acrylamide) beads or with water-soluble, substituted poly(vinyl alcohol) is presented in Scheme 9 the isolated overall yield of lactose was 29.9% (soluble-polymer approach). The synthesis on light-sensitive polymers facilitates the isolation of products, which is important from the preparative point of view and as a tool for the study of enzymes, permitting efficient comparison of acceptor specificity and being capable of demonstrating de novo synthesis. 

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