Inositol, tritiated

In this experimental protocol, the results for the formation of tritiated phosphate inositol are as follows (based on percent of control) InP, 450 InP2, 360 InP3, 270. These results support the conclusion that PAF stimulates the breakdown of phosphoinositides via a phospholipase-C-mediated pathway. 

It has also been shown that, in pollen tubes, both the cell wall and the contents of the Golgi vesicles can be removed by pectinase, or stained with specific, pectin reagents. When these tubes were treated with tritium-labeled mi/o-inositol (which serves as a precursor of D-galacturonic acid in pectin ) and tritiated methyl-methionine (which is a methyl-group donor ), the labeled materials were found first in the Golgi vacuoles. ... 

Arg ]-vasopressin stimulated surfactant secretion in primary cultures of rat type 2 pneumocytes independently of adenosine 3 ,5 -cyclic monophosphate (Brown and Wood 1989). A 50% loss of tritiated phosphatidyhnsositol 4,5-biphosphate (PIP2) occurred from cells prelabeled with myo[ H]inositol within 15 s (Brown and Chen 1990). Consistent with vasopressin-induced PIP2 hydrolysis the two breakdown products, 1,2-diacylglycerol and inositol 1,4,5-triphosphate, was observed. Vasopressin stimulated protein kinase C activity twofold over the basal activity of 0.7410.07 nM/min x mg protein. The [Arg ]-vasopressin antagonist, 1-deamino-8-D-arginine vasopressin, inhibited [Arg ]-vasopressin activation of protein kinase C. 

The tritiated inositol derivatives 92 have been prepared as substrates for T. brucei a-o-GLCpNAc-PI de-A -acetylase, and derivatives 93 as inhibitors of the enzyme. ... 

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