Inositol Phosphorylceramide

Similar to PI species, inositol phosphorylceramide (IPC) species can be readily ionized to form [M-H] in the negative-ion mode. Ionization of IPC species in the positive-ion mode could form [M-i-H] and [M-FLi]+ under certain experimental conditions. However, ionization efficiency to form these ions is much lower in comparison to that of [M-H] [25]. 

The fragmentation pattern of IPC species in the negative-ion mode (i.e., [M-H] ) after low-energy CID is structural informative [25], including the following  

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The principal sphingosylphosphatide component of yeast, fungi, and slime molds is inositol phosphorylceramide (Ins-PCer, compound 3, Fig. 3), which has a phytoceramide or dihydrosphingosine backbone and an inositol phosphate head group. InsPCer is formed in a reaction catalyzed by Ins-... 

Figure 3 The structures of sphingomyelin (1), ceramide aminoethylphosphonate (2), and inositol phosphorylceramide (InsPCer) (3).

Heise N. Characterization of the inositol phosphorylceramide 58. synthase activity of Trypanosma cruzi. Biochem. J. 2005 387 519-529. 

J. M. Figueiredo, W. B. Dias, L. Mendonfa-Previato, J. O. Previato, and N. Heise, Characterization of the inositol phosphorylceramide synthase activity from Trypanosoma cruzi, Biochem. J., 387 (2005) 519-529. 

Biological Applications Measuring activity of a hydrolase studying lipid traffic, membrane traffic inositol phosphorylceramide synthase activity assay ... 

EUiammer, A. Novel assays for inositol phosphorylceramide synthase activity and for inhibitors using fluorescence resonance energy transfer. U.S. Pat. Appl. Publ. US 2007269844, 2007 Chem. Abstr. 2007, 148, 4443. 

Aeed, P. A. Sperry, A. E. Young, C. L. Nagiec, M. M. EUiammer, A. P. Effect of membrane perturbants on the activity and phase distribution of inositol phosphorylceramide synthase development of a novel assay. Biochemistry 2004, 43, 8483-8493. 

Zhong, W. Murphy, D. J. Georgopapadakou, N. H. Inhibition of yeast inositol phosphorylceramide... 

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