Infrared spectroscopy polyacrylamide

Infrared spectroscopy, including FTIR Polyacrylamide gel electrophoresis (PAGE) Amplified ribosomal DNA restriction analysis (ARDRA)... 

Infrared spectroscopy and thermogravimetry have been used in polymer analysis for many years. By coupling the effluent of thermogravimetry to an infrared gas cell, TG/IR (sometimes known as evolved gas analysis) has been used to examine the thermally induced decomposition products a variety of polymers including of poly(vinyl chloride) (7), polyacrylamide (2), tetrafluoroethylene-propylene (3) and ethylene-vinyl acetate (4) copolymers, as well as styrene-butadiene composite (5). 

Despite the sequence identity of PrP and PrP from the same species, they display quite different physical properties. Fourier transform infrared (FTIR) and circular dichroism (CD) spectroscopy reveal that PrP - is essentially a-helical and devoid of P-pleated sheet structure, whereas the P-pleated sheet content of PrP is -40%, while PrP27-30 contains more than 50 % p-pleated sheet. Procedures that destroy the infectivity of PrP27-30 and PrP (e. g. purification by SDS-polyacrylamide gel electrophoresis or treatment with alkali) also substantially reduce the P-pleated sheet content. It therefore appears that the difference between the normal and disease-causing isoforms of PrP is entirely conformational and related to the respective contents of a-helical structure and P-pleated sheet structure. 

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