Inducible promoters, transgene induction

Fig. 3.6 Human serum albumin (HSA) was produced during the sprouting of transgenic rape-seeds. A light-inducible Rubisco promoter was used to control transgene expression. The highest yield was obtained with light induction after three days of continuous darkness. Sprouting was carried out in an airlift fermentor at room temperature. The sprouting medium was water.

Fig. 6. Induction of insect control in transgenic tobacco. The PR-L promoter was fused to the Btk gene and the construct was used to transform tobacco. Both plants are from seeds of a transformed line Bt-13. One plant was sprayed with water and the other with an inducing agent. The plants were challenged after 7 days with Manduca sexta (tobacco hornworm). Control, untransformed plants treated with the inducer do not show insect tolerance. 

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