Indirect immunocytochemistry single 1 antibody

Here then is how it all works together. Immunocytochemistry takes tissue sections or culture cells and incubates them with antibodies. The experimental needs determine the exact order of antibodies incubations and the specific labeling of the antibodies. The general steps in a single primary (1°) antibody indirect immunocytochemistry experiment include the following ... 

Steps in a Single 1° Antibody Indirect Immunocytochemistry Experiment. 106... 

When an experiment uses multiple 1° antibodies derived from the same species, perform the incubations for the first 1° and 2° antibodies, block the remaining antibody sites, and then perform the incubations for the second 1° and 2° antibodies. This procedure consists of a series of two single U antibody indirect immunocytochemistry experiments with extensive blocking between them. The key element is the blocking steps between. In this example, cultures are incubated with the first 1° antibody set, mouse anti-Ag A and 2° antibody goat anti-mouse labeled with 488 fluorophore (Fig. 12.1a), followed by steps that block the remaining antibody sites (Fig. 12.1b, c). The incubations with the second 1° antibody set are with mouse anti-Ag B antibody and 2° antibody goat anti-mouse... 

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