In Vitro Multistep Biocatalysis

Single-step biotransformations have been exploited exhaustively in recent years, and the main emphasis has been on the discovery or/and engineering of new, stable. 

The same group developed a two-step biocatalytic process for e-caprolactone formation, starting from the cheap and easily available raw material cyclohexanol. The desired product was obtained in 94-97% conversion when operating at substrate concentrations in the range 20-60 mM. Additional aspects of the production of e-caprolactone were investigated by Bornscheuer and coworkers [23] by testing different enzyme ratios, coexpression of chaperone proteins 

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