In amyloid plaques

According to the amyloid hypothesis, the A 3 peptide plays a critical role in the pathogenesis of Alzheimer s disease [1]. Major forms of A 3 produced encompass 38, 40 or 42 residues. A 342 is more prone to aggregation than A 340 and in animal models an increased A[342/ A (340 ratio results in amyloid plaque pathology even when total A 3 levels are reduced [4]. The generation of A 3 is a normal process and A 3 is present in the brains and body fluids of humans throughout life. Neuronal... 

Most proteomics analyses involve 2-D electrophoresis and MALDl-TOF MS steps, as this approach allows for a reliable quantification of changes in protein levels. In one study, the LC-MS/MS approach was used for the detection of proteins enriched in amyloid plaques in the AD brain (liao et al., 2004). Employing the former proteomic approach, levels of about 100 proteins were foimd to be changed in the AD brain and cerebrospinal fluid (CSF) in comparison with the control brain and CSF, respectively (Table 15.1). These proteins have various functions, mainly involved in neurotransmission, guidance, signal transduction, metabolism, detoxification, and conformational changes. The CSF proteins are plasma proteins. 

Beta/alpha Enriched in amyloid plaques Liao et al. 2004... 

Using LC-MS/MS, Liao et al. (2004) identified 26 brain proteins enriched in amyloid plaques excised with laser capture microdissection from AD brains. 

Lemere CA, Blusztajn JK, Yamaguchi H, Wisniewski T, Saido TC, SelkoeDJ (1996) Sequence of deposition of heterogeneous amyloid beta-peptides and APO E in Down syndrome Implications for initial events in amyloid plaque formation. Neurobiol Dis 3 16-32. 

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