Improving enzyme activity

In the last decade there were many papers published on the study of enzyme catalyzed reactions performed in so-called chromatographic reactors. The attractive feature of such systems is that during the course of the reaction the compounds are already separated, which can drive the reaction beyond the thermodynamic equilibrium as well as remove putative inhibitors. In this chapter, an overview of such chromatographic bioreactor systems is given. Besides, some immobilization techniques to improve enzyme activity are discussed together with modern chromatographic supports with improved hydrodynamic characteristics to be used in this context. 

Little additional modification to assay conditions should be required. Herpesvirus proteases are active at physiological pH, although the pH optima vary between pH 7.5 (HCMV) and pH 9.0 (EBV). Glycerol has been shown to generally improve enzyme activity. 

Attention then shifted to the 6-position of the pyrone molecule. Variation of the substituents on the phenyl ring at C-6 resulted in a series of derivatives with improved enzyme activity. More specifically, the... 

Amplification of a gene encoding a useful enzyme for improving enzyme activity. 

The effects of the topically applied vitamin E acetate are very well documented in published studies increased moisturization of the homy layer improvement in skin surface relief evident anti-inflammatory properties increased epithelization of surface wounds improved enzyme activity in the skin prevention of skin damage induced by free radicals protection of properties against sunburn. Vitamin E acetate can increase the natural protection of the skin against UV radiation reduce the amount of the UV damaged cells and protect against damage by reactive oxygen radicals. 

---