Improved Immobilization Supports for Candida Antarctica Lipase

Candida antarctica Lipase B (CALB) is atfracting increasing attention as a biocatalyst for the synthesis of low molar mass and polymeric molecules. Almost all publications on immobilized CALB use the commercially available catalyst Novozym 435, which consists of CALB physically adsorbed onto a macroporous acrylic polymer resin (Lewatit VP OC 1600, Bayer). Primarily, commercial uses of CALB are limited to production of high-priced specialty chemicals because of the high cost of commercially available CALB preparations Novozym 435 (Novozymes A/S) and Chirazyme (Roche Molecular Biochemicals). Studies to better correlate enzyme activity to support parameters will lead to improved catalysts that have acceptable price-performance characteristics for an expanded range of industrial processes. [Pg.156]

There are several examples where catalytic active membranes have been developed with immobilized enzymes on a porous ceramic support (Belleville et al, 2001 Lozano et al, 2002). Research on Candida antarctica lipase immobilization has further improved the preparation of active membranes. Another hydrophilic and water-soluble polymer, polyethyleneimine (PEI), which has a high content of free primary aminos as a coating agent, allows for an increase in the number of potential centres for enzymatic attachment (Rios et ah, 2004). [Pg.45]

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