Immunosuppressive therapy cell culture

There are, however, several limitations and contraindications with MSC therapy. One of the limitations is in the establishment of MSC cultures from donors, parents or siblings. Although MSC can be readily isolated and expanded in vitro, establishing the MSC cultures can be a limiting factor. Failure to establish such cell cultures in a timely manner, and in sufficient qualities and quantities, may have profound consequences on the therapy. Since MHC compatibility is not necessary for MSC immunosuppression, and because MSC immunomodulatory activity can also be observed in third-party donor cells [656550], MSCs derived from unrelated, -unmatched, healthy, third-party donors would represent a universal donor MSC product with the advantage of being a readily-available product that may provide an opportunity for multiple and higher MSC doses, potentially at a reduced cost [659019 ]. 

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