Immunostaining muscle cell differentiation

Krenacs, T., Stiller, D., Krenacs, L., Bahn, H., Molnr, F., and Dux, L. (1990) Sarcoplasmic reticulum (SR) Ca +-ATPase as a marker of muscle-cell differentiation immunohistochemical investigations of rhabdomyosarcomas and enhancement of immunostaining after sodium methoxide treatment. Acta Histochem. 88, 159-166. 

S-100 protein (90%) and may be positive with muscle markers such as calponin (86%), desmin (14%), and alpha-smooth muscle actin (36%). 457 Occasional cells exhibit immunostaining with glial fibrillary acidic protein (GFAP). The presence of smooth muscle markers and immunostaining for GFAP is more in keeping with pure myoepithelial differentiation as opposed to... 

Immunostaining studies of cells isolated from the contracting areas within the EBs confirmed the presence of cardiac-specific proteins (MHC, sarcomeric a-actinin, des-min, cTnf, ANP). These studies also demonstrated the presence of early-cardiac morphology with a typical early-striated staining pattern. The cells, however, did not exhibit immunoreactivify with anti-nebuhn monoclonal antibodies (mAbs), a specific skeletal muscle sarcomeric protein shown to be expressed early in skeletal myoblast differentiation. 

Figure 15.4 Printed primary human myoblasts and rat tenocytes to generate muscle-tendon tissues for substance testing. The cells were printed and immunostained for myosin heavy chain (green) in (a) and collagen I (green) in (b) for muscle and tendon tissues, respectively. In (c) the close-up of (a) shows the characteristic muscle striation and multinucleated cell bodies of differentiated myoblasts. Nuclei are stained in blue with (4, 6-diamidino-2-phenylindole). In (d) the close-up of (b) shows the characteristic mature tendon collagen I pattern around the cell nuclei, which are stained in red with propidium iodide. Scale for (a) and (b) = 1 mm. Scale for (c) and (d) = 20 pm.

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