Immunosensors architectures

Figure 9.17 Schematic representation of an immunosensor architecture with AP enzymatic labeling (Reproduced from [124], with permission from Elsevier)...

Brynda E, Houska M (2000) Ordered multilayer assemblies albumin/heparin for biocompatible coating and monoclonal antibodies for optical immunosensors. In Lvov Y, Mohwald H (ed) Protein architecture interfacial molecular assembly and immobilization biotechnology. Dekker, New York... 

Liu and collaborators designed new electrochemical immunosensors based on chemical assembly of vertically aligned SWCNT on carbon substrates for direct detection of the pesticide endosulfan and the insecticide paraoxon " in spiked environmental water samples. In both electrochemical immunosensors, the applied SWCNT forest was fixed onto a GC electrode exploring the formation of amide bonds between the carbo)ylic functional groups at the SWCNT ends and the amine groups on the GC surface, which was previously modified with mixed layers of 4-aminophenyl and phenyl. This architecture was named as GC-Ph-NH2/SWCNT. 

Biosensors based on biorecognition events occurring in monolayer or thin-fihn assemblies on electronic transducers represent an important recent advance in bioelectronics [15]. The nano-architecture of the sensing interface in a mono-layer, multilayer, or thin-fihn structures precludes diffusion barriers, and hence the rapid response-times of the sensing devices are achieved. Enzyme-electrodes [16,17], immunosensors [18,19], or DNA sensors [13] were developed by tailoring nanoscale sensing interfaces on the transducer. We will specifically address the development of DNA sensors in monolayer and thin-film confignrations. 

Figure 9.12 Schematic of the electrochemical Immunosensor assay architecture with 22-(3,5-bis((6-mercaptohexyl)oxy)phenyl)-3,6,9,12,15,18,21-heptaoxadocosanoic acid dithlol PEC-6 carboxylate (DT2) (Reproduced from [123], with permission from Elsevier)...

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