Immunodiffusion tests

Figure 5.9. The single radial immunodiffusion test for antigen quantitation.

In the immunodiffusion test, antibodies against partially punfied GAD also formed a sharp, single band with the purified GAD preparations, whereas the crude preparations showed multiple bands (Matsuda et al, 1973), suggesting that the purified GAD preparation is immunochemically homogeneous... 

The skin, not the blood, is where males perceive the pheromone, however, and since our previous results indicated that the dermis contained abundant lipid, we tested minced dermal tissue in immunodiffusion tests. Initially, we tested dermal tissue of 5 intact untreated sexually attractive females, 2 long-term ovariectomized females, and 2 estradiol-treated females. Only tissue of estradiol-treated females reacted with antivitellogenin tissue of untreated females known to be sexually attractive did not react with anti-vitellogenin. However, untreated tissue did react with adjacent wells containing vitellogenin-positive control solutions. Further, the precipitation line of the reaction between untreated tissue and vitellogenin control was a line-of-identity with the antigen-antibody line and was apparent even in the absence of... 

Immunodiffusion test A serologic test similar to the precipitin test but carried out in agar gel medium. 

Fig. 4. Immunodiffusion test of rabbit antiserum prepared against cellulase CIV with purified enzymes. In center well 10 ul of undiluted antiserum was placed and the outer wells contained each 10 jjl of 3-glucosidase 31 (1.5 x 10 unit) and 3II x 10 unit),...

---