Immunochemical reactions in agar gel

As stated in the introduction, immunoelectrophoresis (abbreviated ImEl) is a two-stage method. The electrophoretic run is comparatively simple and will be dealt with in a following chapter. The immunochemical reaction in agar gel is more complex, and this makes it necessary to examine the advantages of gel difEusion methods as well as their limitations. Many investigators use the Ouchterlony method, preceding or parallel to ImEl. This method will become still more important if present attempts at quantitation gain definite shape. 

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