Immunoassay spectral properties

The number of fluorophores has inaeaaed dramatically during the past decade, and no single source summarizes the diverse spectral properties. Useful information on a wide range of fluorophores can be found in the Molecular Probes catalog, and there is a useful summary of fluoro ( ores used for immunoassays. Unfortunately, detailed information on most fluorophores is only available in the primary literature. 

Organic fluorophores are commonly used as reporters in a large number of in vitro bioassays, such as quantitative immunoassays and fluorescence quenching assays for macromolecular interactions. High sensitivity has been realized with the use of organic dyes, but the spectral properties of QDs could lead to further improvements. Research in the appHcation of QDs for in vitro bioanalysis has been advanced primarily by Mattoussi and his coworkers at the U.S. Naval Research Laboratory [9,10], and can be divided into two areas immunoassays and biosensors. 

In section 9.3.2, we have seen that spectral shifts of incarcerated guests can provide insight into the electronic and spatial characteristics of the inner phase. Of special interest to the photophysical community are also excited state lifetimes of molecules enclosed in constrained media. Investigation of these systems may lead to the development of novel photophysical probes for immunoassays and devices for information storage or solar-energy conversion. Parola et al. investigated the photophysical properties of 9-cyanoanthracene (CA) inside hemicarcerand 26 (Figures 9.3 and 9.34). ... 

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