Immunoassay modern methods

WA Colburn. Radioimmunoassay and related immunoassay techniques, In JW Munson, ed. Pharmaceutical Analysis Modern Methods, Part A. New York Marcel Dekker, 1982, p. 381. 

Chu FS. Immunoassays for mycotoxins. In Cole RJ, ed. Modern Methods in the Analysis and Structural Elucidation of Mycotoxins. New York, NY Academic Press 1986 207-237. 

Modern immunoassay methods employ antibodies immobilized onto solid supports. Antibodies bind strongly and spontaneously to glass and some plastics. They can thus be attached to beads, tubes, columns, or the wells of microtiter plates. These plates, shown in Figure 6.1, consist of 8 rows and 12 columns of wells, and are usually injection molded from polystyrene, to which antibodies and other proteins adsorb irreversibly. Standard dimensions are 86-mm width, 128-mm long, and 7-mm diameter wells, with a volume between 0.3 and 0.4 mL. 

Modern tHcy methods include enzyme immunoassays and chromatographic-based methods. In practice, immunoassays " are most often used for routine purposes (e.g., fluorescence polarization immunoassay IFPIA] as run on Abbott s IMx and AxSYM platforms) Chromatographic assays include amino acid analysis high-performance liquid chromatography (HPLC) with ultraviolet, fluorescence, or electrochemical detection ° " capillary electrophoresis with fluorescence detection gas chromatography-mass spectrometry (GC-MS) and liquid chromatography with tandem MS (MS-MS). 

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