Microchip immunoassay applications

An interesting application of microchip CE is the analysis of serum cortisol immunoassays that have been performed off-chip prior to their injection [64]. Separation and quantitation of the free and bound labeled antigen could be achieved in less than 30 s and allowed the determination of serum cortisol in the clinical range of interest. 

Very recently, von Heeren et al. [59] have performed MECC separations with SCCE. In particular, these authors demonstrated the application of microchip MECC to biologically relevant samples such as labeled urine. A competitive immunoassay for monitoring patient serum levels of theophylline was carried... 

While a wide range of opportunities exist, such as environmental, clinical, and trace analysis, the principal application for labs-on-a-chip is in the analysis of biological samples. The miniaturized dimensions allow extremely small sample volumes to be analyzed, and a microchip format can allow chemical reaction, mixing, sample manipulation, and multiplexing to be performed. Single-cell analysis, immunoassays, protein and peptide separations, DNA analysis and sequencing, and polymerase chain reactions have all been performed on microchip devices [48]. 

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