Immunoassay data reduction

Fig. 8 Data reduction methods to linearize immunoassay concentration-response curves. (From Ref. 647.)...

Fluorometers designed for research purposes(31) are typically equipped with a xenon arc lamp, monochromators, one or more photomultiplier tubes, cuvet holders, and a computer interface. Some research level fluorometers, such as the Perkin-Elmer LS50, have optional microtiter plate reading accessories with fiber optic bundles. This is convenient since 96-well microtiter plates are commonly used for immunoassay development, and many commercial immunoassays are based on the use of microtiter plates. Fluorometers designed for commercial immunoassay purposes are generally dedicated instruments with few, if any, data acquisition and reduction parameters that can be manipulated by the user. 

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