Immunoaffinity chromatography, detection

For detection residue amounts of tetracyclines in dairy products widely used methods FIPLC, immunoaffinity chromatography, kinetic spectrophotometry, which are expensive and complicated. 

Both small and large analytes can be determined using direct detection in lAC. Additionally it is possible to use this technique either separately or in combination with other chromatographic techniques [1]. If this technique is performed as part of HPLC system the method can be referred as high performance immunoaffinity chromatography. 

In the future, one can anticipate increases in sensitivity, specificity and laboratoiy-to-laboratory reproducibility of cost effective and user friendly IA s. Growth will be witnessed in the areas of (i) immunoaffinity chromatography to selectively isolate, enrich or remove components from foods, (ii) immunocytochemistry to localize and understand role of ingredients in foods, (iii) process control with advent of sensors and other on-line detection systems, and (iv) alternate uses. 

Sanchez-Martinez, M., M. Aguilar-Caballos, S. Eremin, et al. 2006. Long-wavelength fluorimetry as an indirect detection system in immunoaffinity chromatography Application to environmental analysis. Anal. Bioanal.Chem. 386 1489-1495. 

Sanchez-Martinez, M.L., M.P. Aguilar-Caballos, S.A. Eremin, et al. 2005. Determination of linear alky-lbenzenesulfonates in water samples by immunoaffinity chromatography with fluorescence detection. Anal. Chim. Acta 553 93-98. 

Braunrath, R., D. Podlipna, S. Padlesak, et al. 2005. Determination of bisphenol A in canned foods by immunoaffinity chromatography, HPLC, and fluorescence detection. J. Agric. Food Chem. 53 8911-8917. 

D.W. Roberts, M.I. Churchwell, F.A. Beland, J.-L. Fang, D.R. Doerge, Quantitative analysis of etheno-2 -deoxycytidine DNA adducts using on-line immunoaffinity chromatography coupled with LC-ESI-MS—MS detection. Anal. Chem., 73 (2001) 303. 

The first publication that reported the use of LC—MS for quantification of IsoPs in urine used reversed-phase LC coupled with ESI/MS. The method used only 1 mL urine and the clean-up procedure using solid phase extraction (SPE) columns gave quantitative recovery of the IsoP. The chromatographic runs and SPE purification methods are short, and this results in a very easy and user-friendly procedure (Li et al., 1999). A comprehensive review by Tsikas et al. describes sample preparation techniques and compares GC—MS methods with the most recent LC—MS/MS methods (Tsikas et al., 2003). The focus is primarily on 8-f5o-PGF2ci and highlights the difficulty to detect only one IsoP isomer without immunoaffinity chromatography preparation. The large concentration differences for the various IsoP classes in urine are also addressed. 

Kawatsu K, Shibata T, Hamano Y. Application of immunoaffinity chromatography for detection of tetrodotoxin from urine samples of poisoned patients. Toxicon 1999 37 325133. 

Luo H, Chen L, Li Z, Ding Z, Xu X. Frontal immunoaffinity chromatography with mass spectrometric detection a method for finding active compounds from traditional Chinese herbs. Anal Chem 2003 75 3994-8. 

Visconti A, Pascale M (1998) Determination of zearalenone in corn by means of immunoaffinity clean-up and high-performance liquid chromatography with fluorescence detection. 

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