Immunoaffinity chromatography antibody-antigen interaction

Immunoaffinity chromatography utilizes the high specificity of antigen—antibody interactions to achieve a separation. The procedure typically involves the binding, to a soHd phase, of a mouse monoclonal antibody which reacts either directly with the protein to be purified or with a closely associated protein which itself binds the product protein. The former approach has been appHed in the preparation of Factor VIII (43) and Factor IX (61) concentrates. The latter method has been used in the preparation of Factor VIII (42) by immobilization of a monoclonal antibody to von WiHebrand factor [109319-16-6] (62), a protein to which Factor VIII binds noncovalenfly. Further purification is necessary downstream of the immunoaffinity step to remove... 

Immunoaffinity chromatography (lAC). Immunoaflinity chromatography, based upon a highly specific biological interaction such as that between antigen and antibody, is a bioanalytical method for separating biochemical mixtures. 

Immunoaffinity column chromatography Antigen-antibody interaction a b [25]... 

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