Proteomics immuno

Celis JE et al. Proteomics and immuno-histochemistry define some of the steps involved in the squamous differentiation of the bladder transitional epithelium a novel strategy for identifying metaplastic lesions. Cancer Res 1999 59 3003-3009. 

The popularity of aptamer-based assays has risen over the last several years in proteomics (25). Typical aptamers are single-stranded nucleic acids that can recognize target molecules. The general basis is similar to immuno-based methods. These aptamers are found by screening large libraries of nucleic acid sequences. This technology has been applied to several proteome-level detections (26-29). 

Saxena, C., Zhen, E., Higgs, R.E., and Hale, J.E. (2008) An immuno-chemo-pro-teomics method for drug target deconvolution. J. Proteome Res. 7, 3490-3497. 

Methods for cell and sub-cellular fractionation include immuno-isolation, electromigration e.g. free flow electrophoresis), flow cytometiy, density gradient isolation of organelles and sequential extraction. The use of some of these approaches to investigate the mitochondrial and myofibrillar sub-proteomes of the heart will be described in Sections 3.4 and 3.6 respectively. A particular problem in proteomic analysis of the heart is the diversity of cell types that are present. Proteomic profiles of total myocardial lysates are dominated by the proteins present in cardiac myocytes, but such samples... 

Sparbier K, Wenzel T, Dihazi H, Blaschke S, Muller GA, Deelder A, Flad T, Kostrzewa M (2009) Immuno-MALDI-TOF MS new perspectives for clinical applications of mass spectrometry. Proteomics 9 1442-1450... 

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