Immobilized proteins multipoint attachment

How does immobilization stabilize a protein First, it reduces the inter-molecular interaction, thus abolishing aggregation (38). Second, it minimizes the unfolding of the protein molecule by multipoint attachment to the matrix. Thus, the number of attachment bonds between the enzyme and the matrix correlates well with the enhancement in stability achieved as a result of immobilization (39). Mozhaev et al. (39) note that this stabilization cannot be pushed beyond a certain limit because irreversible denaturation is always possible. 

Perhaps the most effective method of immobilization for stabilizing a protein is through multipoint covalent bindings between the protein surface and a polymer support. In this way, the protein is covalently attached to a three-dimensional support lattice through multiple points leading to a significant reduction in protein mobility. Enzymes immobilized using this technique far exceed the stability... 

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