PH gradient immobilized

Righetti, P. G., Isoelectric focusing of proteins in conventional and immobilized pH gradients, in Protein Structure A Practical Approach, Creighton, T. E., Ed., IRL Press, New York, 1989, 23. 

Because the pi of a protein is based on its amino acid sequence, this technique has good resolving power. The resolution can be adjusted further by changing the range of the pH gradient. The use of immobilized pH gradient (IPG) strips has enabled reproducible micropreparative fractionation of protein samples, which is not consistently possible when ampholytes are used in the first dimension (Gorg et al., 2000). 

Gorg, A., Obermaier, C., Boguth, G., Harder, A., Scheibe, B., Wildgruber, R., and Weiss, W. (2000). The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21, 1037-1053. 

Ek, K., Bjellqvist, B., Righetti, R G. (1983). preparative isoelectric-focusing in immobilized pH Gradients. 1. General principles and methodology. J. Biochem. Bioph. Meth. 8(2), 135-155. 

Cargile, B.J., Talley, D.L., Stephenson, J.L., Jr. (2004b). Immobilized pH gradients as a first dimension in shotgun proteomics and analysis of the accuracy of pi predictability of peptides. Electrophoresis 25, 936-945. 

Hanash, S.M. (2000). Biomedical applications of two-dimensional electrophoresis using immobilized pH gradients current status. Electrophoresis 21, 1202-1209. 

Norbeck J et al. Two-dimensional electrophoretic separation of yeast proteins using a non-linear wide range (pH 3-10) immobilized pH gradient in the first dimension reproducibility and evidence for isoelectric focusing of alkaline (pi >7) proteins. Yeast 1997 13 1519-1534. 

Righetti, P.G., Immobilized pH Gradients Theory and Methodology, Elsevier, Amsterdam, 1990. 

PI. Pirttila, T., Frey, H., Mattila, K., and Siden, L., Immobilized pH gradient isoelectric focusing of cerebrospinal fluid proteins. Electrophoresis 9, 582-588 (1988). 

O Farrel PH (1975) J Biol Chem 250 4007 Dunn MJ, Burghes AHM (1983) Electrophoresis 4 97 4 173 Gorg A, Postel W, Gunther S (1988) Electrophoresis 9 531 Righetti PG (1990) Immobilized pH gradients theory and methodology. Laboratory techniques in biochemistry and molecular biology, vol. 20, Elsevier, Amsterdam... 

GOrgA(2003) Two-dimensional electrophoresis with immobilized pH gradients for proteome analysis, http //www.weihenstephan.de/blm/deg... 

Gorg A, Postel W, Gimther S. (1988) The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 9, 531 6. 

Haase R, Menke-Mollers I, Oette (1988) Analysis of human apolipoproteins C by isoelectric focusing in immobilized pH gradients. Electrophoresis 9 569-575... 

Guillonneau, F., Labas, V., Auvin, C., and Praseuth, D. (2001) A reliable and simple method for two-dimensional electrophoresis and identification of HeLa nuclear alkaline nucleic acid-binding proteins using immobilized pH gradient. Electrophoresis 22, 4391-4403. 

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