Immobilization-Stabilization of Dehydrogenases

Dehydrogenases are usually multimeric enzymes. Thus, under certain conditions even thermophilic dehydrogenases may be easily inactivated by subunit dissociation, being these enzymes an excellent target for the development of stabilization strategies by immobilization and post-immobilization modification techniques as shown in Fig. 6.4.5. 

As an example, the dehydrogenase from Thermus thermophilus was highly stabilized, as seen in Fig. 6.4.6. 

It is also possible to distort the three-dimensional structure of the enzymes during the multi-point covalent immobilization step with the purpose of altering (improving) its selectivity towards non-natural substrates. 

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