PH gradient immobilised

Cordwell SJ et al. Characterisation of basic proteins from Spiroplasma mellifer-um using novel immobilised pH gradients. Electrophoresis 1997 18 1393-1398. 

Westbrook, J.A., Yan, J.X., Wait, R., Welson, S.Y., Dunn, M.J. (2001). Zooming-in on the proteome very narrow-range immobilised pH gradients reveal more protein species and isoforms. Electrophoresis, 22(14), 2865-2871. 

Principle of the two-dimensional SDS-PAGE and immobilised pH gradient gels... 

Corbett, J. M., M. J. Dunn, A. Posch, and A. Gorg. 1994, Positional reproducibility of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension an interlaboratory comparison. 

Urquhart, B.L., Atsalos, T.E., Roach, D., Basseal, D.J., BjeUqvist, B., Britton, W.L., and Humphery-Smith, I. 1997, Proteomic contigs of Mycohacterium tuherculosis and Mycobacterium bovis (BCG) using novel immobilised pH gradients. Electrophoresis 18 ... 

For good separation results, a stable pH gradient with constant conductivity is required. This can be achieved by carrier ampholytes or immobilised pH gradients. 

Corbett, J., et at. (1994). Positional Reproducibility of Protein Spots in Two-dimensional Polyacrylamide Gel Electrophoresis Using Immobilised pH Gradient Isoelectric Focusing in the First Dimension An Interlaboratory Comparison, Electrophoresis 15 1205 1211. 

Shimao, K., Computer simulation of electrophoretic transport. II. Modified general procedure and application to isoelectric focusing of proteins using immobilised pH gradient, Jap. J. Electrophor., 38, 221, 1994. 

Significant differences are also apparent for the effect of pH on the fermentation rate. The narrow pH optimum characteristic of a free cell system is replaced by an extremely broad range upon immobilisation. This effect stems from the gradient pH that exists within the bead. 

Gradual desorption of the immobilised analytes is achieved either by a continuous increase in ionic strength or a change in pH of the mobile phase. Salt gradients,... 

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